Ardien Consulting Services

Your Partner in Isolator Validation

Founded in 1994 by Rick Nieskes, Ardien Consulting Services provides customized isolator validation to the pharmaceutical industry.  The firm has successfully validated over 100 isolators for production, sterility testing, and containment based applications for over 40 different pharmaceutical companies throughout the world.

D-Value Analysis Service

Ardien Consulting Services D-Value Analysis Apparatus in Progress During a Decontamination Cycle

Ardien Consulting Services D-Value Analysis Apparatus in Progress During a Decontamination Cycle


Based upon numerous requests, Ardien Consulting Services can perform a D-Value Analysis on your isolator with the application of the Halvorson-Ziegler Equation and/or the Limited Holcomb Spearman Karber Method.

The custom designed and fabricated D-Value Apparatus allows for up to 11 biological indicators at each exposure time interval.  Testing is performed easily while stationed in the room surrounding the isolator.

The data obtained from using the D-Value Apparatus can be useful in identifying whether or not your biodecontamination cycle has been properly developed, to gather information as it relates to an investigation following biological indicator positive results in an annual requalification, or to determine the incidence of "rogue" biological indicators.

All work comes with a comprehensive report detailing all results.

If you are interested in this service, simply click here.

Questions?  Email me or call me at 262-853-9748.

To request a quote, click here.

© 2023 Ardien Consulting Services, LLC

Last Update: 11-Jan-2023